The fact that alternative methods do not reduce the number of laboratory animals used is demonstrated by the university district of Zurich where – contrary to the industrial district of Basle – animal experiments can not be transferred to affiliates and partner companies abroad. Thus between 1989 and 2010, the number of laboratory animals used each year in Zurich did not decrease, but increased 95%.
The researchers who develop alternative methods consider animal experiments useful and necessary. Consequently, they work for the most part with cells, tissues and organs obtained from animals killed in laboratories or slaughterhouses, and repeat, for years, the very animal experiments their alternative methods are supposed to replace, to compare them to the alternative methods.
In short, animal experiments are neither reduced, nor replaced, but perpetuated by alternative methods used as a control. Although anti-animal, such methods are ironically financed by misguided animal protection organisations.
Biomedical research based on vivisection is deceptive and misleading, and promoting alternative methods is largely to blame for continued suffering of lab animals.
Instead of truly scientific methods like human cell and tissue cultures and clinical investigations of human patients, alternative methods are based on animal cell, tissue cultures and computer models making them as worthless as the experiments that use animals.
For the validation of alternative methods – a process which takes years, if ever, to complete – researchers compare not only the data from their alternative methods with data from animal experiments, but also repeat the very animal experiments their alternative methods are supposed to replace in order to obtain additional data for the purpose of further comparisons.
Interestingly, it was reported in 2022 that in the first human clinical trial of its kind, a 3D-bioprinted human ear was implanted in a patient suffering from unilateral microtia – a birth deformity where one of the outer ears is malformed or missing – at the Microtia-Congenital Ear Deformity Institute in San Antonio, Texas (USA). The implant was matched to the patient’s healthy ear in shape and size and was made out of ear cartilage cells, sourced from the patient herself. So far ear implants have been reconstructed using synthetic materials, which can be less flexible, or the patient’s rib cartilage, but these require a more invasive surgery tan the new treatment. By combining biosynthetic materials with natural human tissues, 3D printing technology stands at the cutting edge of revolutionary strides in reconstructive medicine.
In December 2023, in order to test new drugs without using animals, using a 3D printer a “body-on-chip” was invented by research scientists of the University of Edinburgh. It mimics how medicine flows and is distributed through five human organs (heart, lungs, kidney, liver and brain) of a patient’s body. In short, it reveals where the new drug goes in the body, how long it stays there and shows the body’s reaction to the drug without having to use an animal or even human.
New Drugs
In August 2015 the Drug Technical Advisory Board (DTAB) decided that “repeat animal testing” was not required for permission for a new drug or clinical trial. Some months later the Ministry of Health (Government of India) amended Schedule Y of the Drugs and Cosmetics Rules, 1945, which states that animals will be spared the cruel tests for new drug registrations where complete data from similar toxicity experiments already exists for drugs approved abroad.
India is a signatory to an international agreement on “mutual acceptance of data” we are obliged to accept the results of such animal testing conducted in other countries and there is no need for the tests to be repeated here. According to the Organisation for Economic Co-operation and Development (OECD) “test data generated in any member country in accordance with OECD test guidelines and principles of good laboratory practice shall be accepted in other member countries.”
Moreover, the Investigating New Drugs committee has in keeping with international trends and practices recommended the use of alternative methods to animal tests. Despite this, in 2017 the Patanjali Research Institute decided to test its medicines on rats and rabbits in the hope that Ayurveda will get recognition in the world of medicine, and unfortunately the Committee for the Purpose of Controlling and Supervising Experiments (CPCSEA) and Animal Ethical Committee gave them permission to do so.
It is very sad that the CPCSEA approve experiments on animals. 12,000 experiments on Beagles (dogs) were approved by them between 2007 and 2011. Of these at least 4,000 dogs were procured for experiments and at least 3,000 either died or were euthanized.
In June 2025, a week after the Telangana Police booked Palamur Biosciences on charges of animal cruelty, the Centre appointed inspection panel called for rescue of 1,200+ animals from the facility citing poor health and living conditions and recommended “immediate regulatory action, including the removal and rehabilitation of animals to prevent further pain and suffering” and a review of the its registration and breeding licence.
The CPCSEA had some years earlier registered Palamur Biosciences which claims to be the most preferred animal testing facility in India. It is a full service pre-clinical contract research organisation. Their services include animal tests for pharma-biotech, agrochemical, MRIDA, research & innovation and laboratory animal research models. They use Beagle dogs, swine, goats and primates under the category of non-rodents; rodents are rats, mice, rabbits and guinea pigs; the avian species used by them are Japanese quail, chicken, pigeons and Mallard ducks; fish are cold water, common carp and Zebrafish; in addition to which they use earthworms, lemna, daphnia, honeybee and silkworms. All suffer following torture. The company’s infrastructure supports Pharma, Medical Devices, Biotech and Pesticide industries.
Experiments (read torture) on Beagles have been undertaken for the following reasons:
65% for toxicity study
11% for dose assessment
10% for tests
9% for pharmakoinetic study
5% for bioequivalence study
After the dogs are no longer needed (old or infirm), a very tiny percentage are handed over to animal welfare societies for adoption.
In November 2020 the Department of Radio Diagnosis and Imaging at the PGI Chandigarh installed a Digital Subtraction Angiography (DSA) facility dedicated for basic (cruel) research on animals. The facility will initially test (read inflict pain, suffering and eventual death) new drugs and hardware that can potentially be used for treatment of human disorders, by evaluation of vessels inside laboratory animal organs like brain, liver, kidneys, etc.
Xenotransplantation
Whereas allotransplantation is the taking of living organs (cells, tissues, etc.) within the same species as in organ donations from human to human, xenotransplantation means taking a living organ from one species and transplanting it into another, like from pigs to humans or baboons to humans.
The history of xenotransplantation as documented:
17th Century: Jean Baptiste Denis began transfusing blood from animals to humans.
1838: The first corneal xenotransplantation from a pig took place – 65 years prior to the first human to human cornea transplant.
19th Century: Skin grafts, mainly from frogs, from animals were used on humans in Europe.
1920s: Serge Voronoff, a Russian scientist began testicular transplants from chimpanzees to aging men but his work was later discredited.
1963: Keith Reemtsma, a US doctor, transplanted chimpanzee kidneys into 13 humans. All failed between 4 to 8 weeks except for one who lived for 9 months.
1964: James Hardy, a US surgeon transplanted a Chimpanzee’s heart into a human but the patient died in 2 hours.
1983: Leonard Bailey of the US transplanted a baboon heart in an infant called Baby Fae who survived for 20 days only. (See below.)
1992: Dr Jonathan Ho Kei-Shing fit heart valves made from ox tissue (designed by Dr Baruah) into human patients.
1997: Dr Dhani Ram Baruah of Sonapur, near Guwahati illegally transplanted a pig heart into a human. The recipient survived for a week only.
Both Dr Ho and Baruah were arrested.
2021: New York surgeons attached a kidney from a genetically modified pig to a brain-dead man and watched as it began to function. Shortly thereafter the University of Alabama announced a similar procedure with similar results.
2022: Doctors at the University of Maryland Medical Centre, USA, transplanted a pig heart into a patient citing organ shortage crisis due to the pandemic. This was repeated but both the patients died.
In June and July 2022 a team of researchers at NYU Langone’s Tech Hospital transplanted two genetically engineered pig hearts into recently deceased humans and monitored their heart function for 3 days to check for signs of early rejection.
2024: In March kidney from a genetically engineered pig was transplanted by surgeons in Boston into an ailing living man. Earlier such kidneys had only been transplanted into brain-dead patients.
In April 2024 doctors at NYU Langone Health implanted a mechanical pump to keep a patient’s heart beating and 8 days later transplanted a kidney from a GM pig. (It was then stated that more than 100,000 were on the transplant waiting list, most of them needing a kidney.)
Now pig heart valves are routinely transplanted into human patients. However, since bovine organs are too big to transplant in humans, tissues have been used to repair cardiac defects and reconstruct heart valves. In this way, cow body parts have for decades been transplanted in humans abroad and in India too – in July 2015 at Chennai an 81-year old Hyderabad woman’s leaking heart valve was replaced with a bioprosthetic heart valve made from a cow’s heart tissues.
It all began with using pig skin for extensive burns, and went on to transplanting the heart of a baboon in a child known as Baby Fae (mentioned above) who did not survive because the baboon and she were of different blood groups. (If there was even a shred of ethics in those who undertook this experiment, they would have checked the blood groups before killing the baboon and ruled against the transplant.) Luckily for baboons they very rarely have the universal blood group O so the possibility of using their body-parts has dropped.
Few know that in 2009 six persons were arrested in a fake blood racket – animal blood was mixed with that of humans – in Lucknow which had been going on since 2005 under the seal of a medical college.
In 2016 the National Institute of Health, USA, proposed a new policy which would allow researchers to receive federal funds to make part human, part animal embryos. In effect crossbreeding of humans and animals could be undertaken although not approved as such!
The first human-pig embryo was grown in a laboratory in 2016. Scientists of the Salk Institute for Biological Studies in La Jolla, California announced that they had created human-pig hybrids. The pig embryos were injected with human stem cells and implanted into sows and left to grow. Of 2,075 embryos, only 186 developed to the 28 day stage. In other words biologists grew human stem cells in a pig’s embryo. The approach involved generating stem cells from a patient’s skin for growing the desired new organ in a pig and then harvesting it for transplant into the patient’s body, and since the organ would be of the patient’s own cells there would be little risk of immune rejection. Creatures composed of two different genomes as in this case of human-organ-growing pigs, are called chimeras.
Another group of scientists from Sanford University showed that a mouse pancreas grown this way in a rat, which then had parts of it transplanted into a mouse genetically identical to the one that supplied the stem cells employed, could control diabetes in that mouse, thus creating a working, transplantable organ. The group followed up their mouse work by growing human pancreas cells in pig fetuses. Similarly scientists from the University of California have managed the same in sheep.
Pigs are being exploited the most. After editing their genes (eliminating porcine endogenous retroviruses abbreviated as PERVs) they will be specially bred for xenotransplantation. So GM kicks in. In 2017 researchers at Harward via gene-editing followed by cloning of these edited cells, created piglets cleansed of viruses that cause disease in humans. But cloning fails often: in this instance they created 30 piglets (i.e. 8 litters of PERV-free piglets) of which most of the embryos and fetuses died before birth, whereas some died soon after they were born. Few survived 4½ months said to be the ideal age to “harvest” their organs for xenotransplantation. Thus the scientists hope that one day they will make it possible to easily grow and transplant livers, hearts and other organs from pigs into humans.
At the 2018 annual meeting of the American Association for the Advancement of Science in Austin, Texas, a panel of researcher came up with the following procedure: “Take the fertilised egg of a pig. From each cell in the resulting embryo cut out a gene or genes that promote the development of the animal’s heart. Inject human stem cells from a patient who needs a new heart into the embryo and then place it into the womb of a sow. Wait nine months. The result is an adult pig with a heart made of human cells. The pig can be slaughtered and the heart transplanted into the patient who provided the stem cells, for whom the organ will be a genetic match.” Moreover they feel that sheep and cows can also host human organs and since the animals are already raised for their flesh and skin, their use to grow more valuable things should meet with no objection beyond squeamishness.
>In fact, since April 2015 pig corneas are being used in China under the brand name of Acornea. Initial trials were conducted using tissue from chickens, cows, ducks, geese, monkeys and sheep, but pig tissue was favoured because bio-mechanical properties of human and pig corneas are very similar.
It was reported in 2022 that a team of international researchers including from the All Indian Institute of Medical Sciences India, had developed an implant made of collagen protein from pig’s skin, which resemble the human cornea and restored vision. In a study published in Nature Biotechnology the implant restored vision to 20 persons in India and Iran who were blind prior to receiving the implant.
In 2016 South Korean researchers of the National Institute of Animal Science reported they had successfully installed a pig’s heart in a monkey. The crab-eating macaque was also given a cornea from the pig’s eye. The pig had been genetically engineered in 2010 to produce an excessive amount of a membrane protein that helps reduce the risk of the organ being rejected after transplantation.
In 2017 a “synthetic soft-tissue retina” was developed by researchers at the Oxford University. It consisted on water droplets (hydrogels) and biological cell membrane proteins (presumably of animal origin). It was due to be tested on animals.
In January 2019 it was reported that at the Institute of Neuroscience of the Chinese Academy of Sciences 5 multiple clones from a gene-edited monkey for biomedical research were born and that they had been induced with human diseases like sleep disorder, mental problems, depression, anxiety and behaviour linked to Schizophrenia. A month later, following the gene-editing uproar, China drafted new rules to supervise biotechnology research that included fines and bans against rogue scientists.
Despite this, in July 2019 China produced its first cloned cat. $35,000/- was paid by the owner who ordered it. Duplicating a dog costs around $ 53,000/-. The animal looks the same, but would the soul be the same? In any case, it is totally unethical.
Around the same time British scientists used CRISPR technology to develop gene-edited chickens designed to be totally resistant to flu. The transgenic chicks will be hatched later in 2019 at the Roslin Institute at the University of Edinburgh in Scotland. (Roslin Institute was responsible for creating the world’s first cloned animal, Dolly the sheep.)
In August 2019, the 87 years old Sir Terence English who had performed the first heart transplant in the UK in 1979 declared that his team would this year transplant a pig’s kidney into a human’s body.
In January 2022 Dr Bartley Griffith the lead surgeon at the University of Maryland Medical Centre, USA, transplanted a pig heart into a patient citing organ shortage crisis due to the pandemic. The recipient survived for only 2 months post-surgery and it was suspected tat the patient died of a viral infection in the pig’s heart. In June and July 2022 a team of researchers at NYU Langone’s Tech Hospital transplanted two genetically engineered pig hearts into recently deceased humans and monitored their heart function for 3 days to check for signs of early rejection.
The ICMR (Indian Council of Medical Research) guidelines only permit transplants between animals, not xenotransplantation. BWC hopes the Congress of the Asian Society of Transplantation will not recommend xenotransplantation upon discussing it at their conference in New Delhi in September 2019.
Who all does Man playing God benefit? Scientists, researchers, drug companies, hospitals, health care, media, and so on – all financially gain with business and fame as a bonus. The donor (animal) is killed. The donee (human) suffers and eventually dies.
BWC strongly opposes killing animals for their body parts because their lives are as sacred as human lives.
Humane Heart Tissue and other Transplants
Researchers at Massachusetts’ Worcester Polytechnic Institute are developing a method to use the vascular network in spinach leaves to deliver blood, oxygen and nutrients to grow human tissue.
In 2017 Chinese doctors grew in three months a new ear on a man’s arm and successfully transplanted it. The plastic surgeon Guo Shuzhong of the First Affiliated Hospital of Xi’an Jiaotong University and his team first stretched the man’s skin on his arm with a skin expander. Then they took a piece of cartilage from one side of his chest to carve out a new ear before planting the artificial organ on the patient’s forearm.
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